Isorhodopsin rather than rhodopsin mediates rod function in RPE65 knock-out mice
نویسندگان
چکیده
منابع مشابه
Isorhodopsin rather than rhodopsin mediates rod function in RPE65 knock-out mice.
The chromophore of visual pigments is 11-cis-retinal and, thus, in its absence, opsin is not photosensitive and no visual function exists. However, in the RPE65 knockout (Rpe65-/-) mouse, where synthesis of 11-cis-retinal does not occur, a minimal visual response from rod photoreceptors is obtained. We have examined if an alternative pathway exists for cis-retinoid generation in the absence of ...
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The visual pigment content of rod photoreceptors in Xenopus larvae was reduced greater than 90% through a combination of vitamin A-deficient diet and constant light. Thereafter, a dose of either all-trans-retinol or 9-cis-retinal was injected intramuscularly, leading to the formation of a rhodopsin (lambdamax 504 nm) or isorhodopsin (lambdamax 487-493 nm) pigment, respectively. Electrophysiolog...
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......................................................................................................................................... ii Acknowledgements ...................................................................................................................... iii Table of
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The visual process is initiated by the photoisomerization of 11-cis-retinal to all-trans-retinal. For sustained vision the 11-cis-chromophore must be regenerated from all-trans-retinal. This requires RPE65, a dominant retinal pigment epithelium protein. Disruption of the RPE65 gene results in massive accumulation of all-trans-retinyl esters in the retinal pigment epithelium, lack of 11-cis-reti...
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The preparation and properties of three geometric isomers of 13-desmethyl retinal (13-dmr) are described. They are analogous to the all-trans, 11-cis, and 9-cis isomers of retinal since two of the cis isomers combine with cattle opsin to form pigments which are spectrally indistinguishable from rhodopsin and isorhodopsin and the all-trans isomer is unreactive.The pigment which resembles rhodops...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2003
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.2234461100